Un article de l’équipe “Hélicases & ARN : mécanismes, ciblage et bio-mimétisme” vient de paraître dans la revue “The EMBO Journal” :
The Sm-like RNA chaperone Hfq mediates transcription antitermination at Rho-dependent terminators –
Makhlouf Rabhi, Olivier Espéli, Annie Schwartz, Bastien Cayro, A Rachid Rahmouni, Véronique Arluison and Marc Boudvillain –
Advance online publication 14 June 2011; doi:10.1038/emboj.2011.192 –
In Escherichia coli, the essential motor protein Rho promotes transcription termination in a tightly controlled manner that is not fully understood. Here, we show that the general post-transcriptional regulatory protein Hfq associates with Rho to regulate Rho function. The Hfq:Rho complex can be further stabilized by RNA bridging both factors in a configuration that inhibits the ATP hydrolysis and duplex unwinding activities of Rho and that mediates transcription antitermination at Rho-dependent terminators in vitro and in vivo. Antitermination at a prototypical terminator (λtR1) requires Hfq binding to an A/U-rich transcript region directly upstream from the terminator. Antitermination is modulated by trans-acting factors (NusG or nucleic acid competitors) that affect Hfq association with Rho or RNA. These data unveil a new Hfq function and a novel transcription regulatory mechanism with potentially important implications for bacterial RNA metabolism, gene silencing, and pathogenicity.
“Imaging of diagnostic and therapeutic biomarkers in Oncology” Workshop.
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