Everything you’ve always wanted to know about protein/protein interactions and kinase activity assays is in a paper published in JOVE.

11 July 2019 par Isabelle Frapart
A video paper has just been published in JOVE, Journal of Visualized Experiments, a peer-reviewed scientific journal that publishes experimental methods in video format. It focusses on basic technics in Biochemistry to unravel protein interaction by coimmunoprecipitation experiments and to assess their kinase activity.

"Cell signalling" group led by the Dr H. Bénédetti has published a video paper in JOVE, Journal of Visualized Experiments, a peer-reviewed scientific journal that publishes experimental methods in video format.

This paper belongs to the Method section focusing on basic technics in Biochemistry.

Different experiments are detailled:

- Transient cell transfections
- Protein extraction
- Study of protein/protein interaction by co-immunoprecipitation experiments
- Study of kinase activity by g32P-[ATP] labeling or by using phospho-specific antibodies

All these different technics are illustrated with data obtained by the group on LIMK2-1, a new protein the team has just pointed out and characterized. LIMK2-1 protein does exist, and it is very atypical in the way it regulates actin cytoskeleton remodeling.

The thematic group "Cell signalling" has published a video paper in JOVE, Journal of Visualized Experiments, a peer-reviewed scientific journal that publishes experimental methods in video format.

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