Nucleotide analog interference mapping (NAIM) is a combinatorial approach that probes individual atoms and functional groups in an RNA molecule and identifies those that are important for a specific biochemical function. Here, we show how NAIM can be adapted to reveal functionally important atoms and groups on RNA substrates of helicases. We explain how NAIM can be used to investigate translocation and unwinding mechanisms of helicases and discuss the advantages and limitations of this powerful chemogenetic approach.
A lire aussi
2025, May 16: seminar of Mickaël Baqué
29 April 2025
par Laetitia Legoupil
A butterfly peptide to combat resistant fungi
16 April 2025
par Laetitia Legoupil
Marcin Suskiewicz, winner of funding from the Human Frontier Science Program, a prestigious international organisation
16 April 2025
par Laetitia Legoupil