- Cell culture (mammals, insects, yeasts, bacterias)
- Flow cytometry, immunolabelling, cell cycle, calcium flow, cell viability, apoptose…
- Confocal microscopy, spectral imaging, static and dynamic videomicroscopy
- Endocytosis and intracellular traffic, membrane permeabilization induiced by par des fusiogenic peptides
- Cell transfection (DNA, mRNA, oligonucleotides)
- Bioluminescence : mesure of luciferase expression
- Molecular biology (cloning, plasmids construction, plasmids and messenger RNA preparation), Northern blot, PCR and real time quantitative PCR, in-situ fluorescence hybridization
- Hypoxia and its control
- Ultraviolet irradiation (UVA, UVB, UVA+UVB)
- Expression and purification of recombinant proteins (mammalian cells, insects, yeasts, bacterias)
- Mutagenesis
- Biochemistry : protein purification, SDS-PAGE gels, Western-blot, immunoprecipitations.
- Purification and characterisation of proteins et glycoproteines
- Chemoenzymatic glycosylations (oligosaccharides, glycopeptides and glycoproteins synthesis)
- Glycans analysis
- Genetic of the yeast Saccharomyces cerevisiae
- Protein-protein interactions , study by : double-hybrid, co-purification, co-immunoprecipitation, Surface Plasmon Resonance, TAP-tag.
- Microinjection
- Fluorescence spectroscopy
- Liposomes préparation
- Synthesis of polymers
- Size and zeta potential of nucleic acids / synthetic vectors complexes