Research topics
Collaborations
Academic partners
Dr D Bagnard, INSERM 1119 BMNTS Lab (Biopathology of Myelin, Neuroprotection & Therapeutic strategy, Strasbourg)
Pr A. Bojarski, Maj Institute of pharmacology (Polish Avademy of Sciences, Krakow, Poland)
Dr S Chalon, INSERM UMR 1253, iBrain (Imagerie et Cerveau, Tours)
Dr R P Gomariz, facultad de biología complutense (Spain, Madrid)
Pr E Kellenberger, Dr F Bihel, CNRS UMR 7200 (Therapeutic Innovation Laboratory, Strasbourg)
Dr C Raoul, INSERM U1051 (Institut des Neurosciences, Montpellier)
Dr H Rebholz, INSERM U1266 (Institute of Psychiatry and Neuroscience, Paris)
Dr E Reiter, INRAE UMR 7247 (Physiology of reproduction and behavior, Tours)
Pr F Suzenet, UMR7311 (Institute of Organic and Analytical Chemistry, Orléans)
Dr E.Traiffort, INSERM U1195 (Diseases and hormones of the nervous system, Kremlin Bicêtre)
Dr S Watts, University of Michigan, Department of Pharmacology and Toxicology, USA
Hospital partners
Dr. Canan OZSANCAK, Dr. Pascal AUZOU, Dr. Maud Pallix-Guyot: Neurology Department, Orléans Regional Hospital (CHRO)
Industrial partners
MabSilico, Synthelis, NB PharmaTox
Partners and backers
ANR, FEDER, Région Centre Val de Loire, Ligue contre le cancer, ARSLA, FRM
Techniques/tools/expertises
Molecular biology
- Cloning
- Directed mutagenesis
- Gene expression by RT-qPCRs
- Phage display screening
Cell culture
- Glial and neuronal cell lines
- Primary culture of neurons
- Transfection
- Recombinant protein production
- Cell signaling (WB, Alphascreen, BRET biosensors)
- Cell sorting
- Cell dynamics (internalization and intracellular traffic) using video microscopy
- Immunocytochemistry
Tissue analysis
- Gene expression: RT-qPCR
- Histology, Immunofluorescence
Animal experimentation
- Disease models: multiple sclerosis, Parkinson's (in collaboration), inflammatory pain
- Behavioral tests
- Pharmacological studies (PK, toxicology)
Main publications:
- Chayma El Khamlichi, Flora Reverchon, Nadège Hervouet-Coste, Elodie Robin, Nicolas Chopin, Emmanuel Deau, Fahima Madouri, Cyril Guimpied, Cyril Colas, Arnaud Menuet, Asuka Inoue, Andrzej J. Bojarski, Gérald Guillaumet, Franck Suzenet, Eric Reiter and Séverine Morisset-Lopez. Serodolin, a β-arrestin–biased ligand of 5-HT7 receptor, attenuates pain-related behaviors. PNAS, 20 mai 2022, 119(21)e2118847119https://doi.org/10.1073/pnas.2118847119/li>
- Anthony Guillemain, Yousra Laouarem, Laetitia Cobret, Dora Štefok, Wanyin Chen, Solal Bloch, Amina Zahaf, Lauren Blot, Flora Reverchon, Thierry Normand, Martine Decoville, Catherine Grillon, Elisabeth Traiffort and Séverine Morisset-Lopez. LINGO family receptors are differentially expressed in the mouse brain and form native multimeric complexes. FASEB Journal (2020) 34 (10): 13641-13653. doi:10.1096/fj.202000826R ⟨hal-02943248⟩
- El Khamlichi C, Reverchon-Assadi F, Hervouet-Coste N, Blot L, Reiter E, Morisset-Lopez S. Bioluminescence Resonance Energy Transfer as a Method to Study Protein-Protein Interactions: Application to G Protein Coupled Receptor Biology, Molecule (2019) 24 (3): 537.
- W Deraredj Nadim, S Chaumont-Dubel, F Madouri, L Cobret, M L De Tauzia, H Bénédetti, P Marin and S Morisset-Lopez. Physical Interaction between Neurofibromin and Serotonin 5-HT6 Receptor Promotes Receptor Constitutive Activity. Proc Natl Acad Sci U S A, (2016) 113 (43): 12310-12315.
- Cobret L, De Tauzia M, Ferent J, Traiffort E, Hénaoui I, Godin F, Hélène Bénédetti and Séverine Morisset-Lopez. Targeting the cis-dimerization of LINGO-1 with small-molecule affects its dowstream signaling. Br J Pharmacol, (2015) (172): 841-856.