This project entitled "Preclinical Validation of LIMK Inhibitors in Cancer" will receive specific financial support. It is led by Hélène Benedetti, a researcher at the Center for Molecular Biophysics (CBM), and Sylvain Routier, a researcher at the Institute of Organic and Analytical Chemistry (ICOA). Its goal is to determine the efficacy of inhibitors, protein kinases LIMKs, new therapeutic targets particularly interesting to treat cancers for which existing therapies are ineffective. Prematuration will make it possible to characterize the cellular effect of these compounds and to determine their pharmacokinetic properties with a view to performing in vivo tests on three cancer models.
Evaluating the activity of compounds on the skin and understanding their mechanisms of action have become unavoidable steps to select and develop a dermocosmetic product. Since the ban on the use of animals in the cosmetic field, many "models" have been developed, especially with recent advances in research in skin biology and computer science.
The book "Modèles pour l'évaluation des produits cosmétiques, de la molécule à l'humain", coordinated by Catherine Grillon, Leader of the Skin Biology Group at CBM, and Marek Haftek of the Laboratory of Tissue Biology and Therapeutic Engineering de Lyon, gives an overview of all the models used to evaluate the activity of dermocosmetic compounds, with their advantages, disadvantages and limitations. This book, edited by Cosmetic Valley Editions, brings together academic and industrial researchers, all specialists in their field, ranging from in silico prediction to in vivo evaluations, to all in vitro cell models, from the simplest to the most complex.
"Cell signalling" group led by the Dr H. Bénédetti has published a video paper in JOVE, Journal of Visualized Experiments, a peer-reviewed scientific journal that publishes experimental methods in video format.
This paper belongs to the Method section focusing on basic technics in Biochemistry.
Different experiments are detailled:
- Transient cell transfections
- Protein extraction
- Study of protein/protein interaction by co-immunoprecipitation experiments
- Study of kinase activity by g32P-[ATP] labeling or by using phospho-specific antibodies
All these different technics are illustrated with data obtained by the group on LIMK2-1, a new protein the team has just pointed out and characterized. LIMK2-1 protein does exist, and it is very atypical in the way it regulates actin cytoskeleton remodeling.
One of the sessions focused on the activity report of the LIA (International Associated Laboratory) between the Laboratory of Molecular Oncology and Innovative Therapies and the Center for Molecular Biophysics, one of the best examples of successful long-term Franco-Polish cooperation.
The association of doctoral students of the Center for Molecular Biophysics (ADOC) is organizing its 17th scientific symposium on Friday, July 5, 2019, whose theme is "Science Together".
This conference will take place in the amphitheater Charles Sadron CNRS.
PhD students and post-docs are invited to present their work during a poster session. To be sent an abstract before June 25, 2019, preferentially in English (French tolerated), as well as the title and the names of the authors at the following address: email@example.com