"Cell signalling" group led by the Dr H. Bénédetti has published a video paper in JOVE, Journal of Visualized Experiments, a peer-reviewed scientific journal that publishes experimental methods in video format.
This paper belongs to the Method section focusing on basic technics in Biochemistry.
Different experiments are detailled:
- Transient cell transfections
- Protein extraction
- Study of protein/protein interaction by co-immunoprecipitation experiments
- Study of kinase activity by g32P-[ATP] labeling or by using phospho-specific antibodies
All these different technics are illustrated with data obtained by the group on LIMK2-1, a new protein the team has just pointed out and characterized. LIMK2-1 protein does exist, and it is very atypical in the way it regulates actin cytoskeleton remodeling.
