Everything you’ve always wanted to know about protein/protein interactions and kinase activity assays is in a paper published in JOVE.

"Cell signalling" group led by the Dr H. Bénédetti has published a video paper in JOVE, Journal of Visualized Experiments, a peer-reviewed scientific journal that publishes experimental methods in video format.

This paper belongs to the Method section focusing on basic technics in Biochemistry.

Different experiments are detailled:

- Transient cell transfections
- Protein extraction
- Study of protein/protein interaction by co-immunoprecipitation experiments
- Study of kinase activity by g32P-[ATP] labeling or by using phospho-specific antibodies

All these different technics are illustrated with data obtained by the group on LIMK2-1, a new protein the team has just pointed out and characterized. LIMK2-1 protein does exist, and it is very atypical in the way it regulates actin cytoskeleton remodeling.

Extraterrestrial organic matter that fell on Earth 3.3 billion years ago

The meteorites bring it regularly, but it has left no trace of it. It has been identified and analyzed by researchers from the CNRS, Chimie ParisTech, the Universities of Tours and Lille. Their publication, in the journal Geochimica and Cosmochimica Acta, offers a first model to distinguish these molecules from elsewhere from those produced on Earth.

References of the article:

D. Gourier et al.
Extraterrestrial organic matter preserved in 3.33 Ga sediments from Barberton, South Africa.
Geochimica and Cosmochimica Acta - May 2019 - DOI: 10.1016 / j.gca.2019.05.009

See article

See the CNRS press release


A new interpretation of neutron scattering spectra

The article presents a new interpretation of neutron scattering spectra by molecular systems that has much in common with the Franck-Condon theory describing the vibrational transitions in a molecule after absorption or emission of a photon. The principal elements are the quantum probabilities for the transitions between the energy levels of the studied system, which are induced by diffusion of a neutron. In this case, the fundamental concept of "energy landscapes", which was introduced by Hans Frauenfelder to describe the internal dynamics of proteins in terms of "jumps" between the minima of their (free) internal energy, can be integrated in the analysis of neutron scattering spectra by complex systems in general. The theory also provides an intuitive physical interpretation of Van Hove's correlation functions in the quantum regime, as well as their classical limit, which is usually considered in the analysis of quasi-elastic spectra of neutrons from proteins and other complex molecular systems.

CBM researchers have created the first method for detecting non-covalent complexes of biomolecules by MALDI mass spectrometry based on liquid deposits

The success of this method relies on the use of nonvolatile liquid matrices, which avoids the passage through the solid phase conventionally used in MALDI and the denaturation of the non-covalent assemblies. By their increased viscosity, these matrices also have the advantage of better mimicking the cluttered environments of living organisms.
The reliability of this method has been shown for non-covalent protein protein and ligand protein systems. This new approach could be used for screening of therapeutic protein ligands and facilitating the analysis of membrane protein complexes by mass spectrometry.

The first computational method to predict Rho-dependent termination of transcription.

Rho-dependent termination is a specific bacterial mechanism, which plays a major role in gene expression and maintenance of genomic integrity. This is an important mechanism for the fast adaptation of bacteria to environmental changes or stresses. Although Rho-dependent termination sites are very diverse and without a real consensus sequence, CBM researchers have identified dozens of quantitative sequence descriptors (eg% C and% G) that, taken collectively, provide good prediction of the sites of Rho action in the model genomes of Escherichia coli and Salmonella (85% success rate).

This work was published in the journal Nucleic Acids Research.