This group investigates structure-function relationships in RNA-based biological systems. Research is focused on RNA remodelling proteins (helicases, chaperones). The group notably develops sophisticated biochemical and bioinformatics approaches to decrypt the molecular mechanisms of these essential factors.
Research theme
The group explores the mechanisms by which the structures of RNA molecules and ribonucleoproteic (RNP) complexes are remodelled. These structures are often dynamic and can evolve within the cell where they govern the role and fate of RNA species. Two main classes of ubiquitous proteins –RNA chaperones and RNA helicases- shape and remodel the structures of RNA and RNP complexes. The dysfunction of endogenous RNA remodelling proteins or the action of viral or bacterial ones are implicated in diverse pathologies including cancers. We study how RNA helicases and RNA chaperones work and cooperate to drive key physiological functions.
RNA chperones usually disrupt secondary structures upon their binding to RNA and promote the formation of alternative structural motifs and/or complexes. RNA helicases use the energy derived from their NTPase activity to disrupt double-stranded RNA, RNA-DNA heteroduplexes, or RNP complexes. These activities are sometimes linked to directional translocation of the helicase along RNA.
To study RNA remodelling proteins, we use complementary biological, biochemical, and biophysical approaches. We focus our efforts on a complex model system wherein Rho, a ring-shaped RNA helicase, is involved in transcription termination and genome maintenance in bacteria.
We study the molecular mechanisms governing Rho activity, how the helicase is involved in gene silencing mechanisms implicating non-coding RNAs and RNA chaperones (Hfq, CsrA), the importance of Rho for bacterial pathogenicity and fitness, how the functions and modes of action of Rho are conserved within the biodiversity, etc.
We are also investigating other, distinct RNA helicase specimens. In this way, we hope to understand the basic mechanisms that govern these important enzymes, determine their key common features and differences, and identify factors that contribute to their regulation.
Our long-term goal is to exploit this information for biomedical purposes and for the design of biosensors or inducible gene circuits useful for synthetic biology.
Significant publications :
- Cédric Nadiras, Eric Eveno, Annie Schwartz, Nara Figueroa-Bossi, Marc Boudvillain.
A multivariate prediction model for Rho-dependent termination of transcription.
Nucleic Acids Research (2018) 46, 8245-8260 - Odil Porrua, Marc Boudvillain, Domenico Libri.
Transcription Termination: Variations on Common Themes.
Trends in Genetics (2016) 32, 508-522. - Emilie Soares, Annie Schwartz, Marcello Nollmann, Emmanuel Margeat, Marc Boudvillain.
The RNA-mediated, asymmetric ring regulatory mechanism of the transcription termination Rho helicase decrypted by time-resolved nucleotide analog interference probing (trNAIP).
Nucleic Acids Research (2014) 9270-9284. - Nara Figueroa-Bossi, Annie Schwartz, Benoit Gillemardet, François D’Heygère, Lionello Bossi, Marc Boudvillain
RNA remodeling by bacterial global regulator CsrA promotes Rho-dependent transcription termination
Genes & Development (2014) 28, 1239-51. - Rachid Menouni, Stéphanie Champ, Léon Espinosa, Marc Boudvillain, Mireille Ansaldi
Transcription termination controls prophage maintenance in Escherichia coli genomes
Proc. Natl. Acad. Sci USA (2013) 110, 14414-14419.
- Filtres
Publications (Archives Hal)
Contact
Disciplines
- Sciences du Vivant [q-bio]35
- Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]11
- Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]/Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire11
- Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]/Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire/Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie structurale [q-bio.BM]10
- Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire7
- Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie structurale [q-bio.BM]/Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire/Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]6
- Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie structurale [q-bio.BM]/Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire6
- Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie structurale [q-bio.BM]6
- Sciences du Vivant [q-bio]/Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire5
- Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire/Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]2
- Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire2
- Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologie/Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]/Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie structurale [q-bio.BM]1
- Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologie/Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]1
- Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologie1
- Sciences de l'ingénieur [physics]1
Mots-clefs
- Rho5
- Transcription termination5
- Helicase4
- ESCHERICHIA-COLI RHO3
- Hexamer3
- Molecular motor3
- RNA3
- STRUCTURAL BASIS2
- Termination2
- Transcription2
- ACTIVATION1
- ADDUCTS1
- APOPTOSIS1
- ATP-BINDING DOMAIN1
- ATPASE ACTIVATION1
- BACTERIOPHAGE PHI-61
- BINDING1
- Bacteroidetes1
- CIS1
- CIS-DIAMMINEDICHLOROPLATINUMII1
- CISPLATIN1
- CONFORMATIONAL-CHANGES1
- CRO MESSENGER-RNA1
- ChiP1
- DEFECTIVE-MUTANTS1
- DEPENDENT TERMINATION1
- DNA1
- DNA HELICASE1
- DNAB HELICASE1
- DRUG1
- ELONGATION COMPLEX1
- FORM1
- Fluorescence1
- HEPATITIS-C VIRUS1
- HEXAMERIC HELICASE1
- HEXAMERIC HELICASES1
- Hexameric helicase1
- Hexameric helicases1
- Hofmeister series1
- IDENTIFICATION1
- IN-VITRO1
- MOLECULAR MOTOR1
- Mechanism1
- Noncoding small RNA1
- NusG1
- P41
- PACKAGING MOTOR1
- PLATINUM ANTITUMOR COMPLEXES1
- POLYMERASE COMPLEX1
- PROTEIN1
- PROTEIN-RHO1
- Packaging1
- Processivity1
- RNA folding1
- RNA helicase1
- RNA metabolism1
- RNA synthesis1
- RNA-BINDING DOMAIN1
- RNA-DNA HELICASE1
- Rho factor1
- Rho protein1
- Rho-dependent termination1
- Ring shaped1
- Rut1
- SECONDARY STRUCTURE1
- STABILITY1
- Transcription termination factor1
- Translocation1
- UNZIPPING MECHANISM1
- Virus TRANSCRIPTION TERMINATION FACTOR1
- X-ray crystallography1
Auteurs
Boudvillain Marc27
Schwartz Annie20
Boudvillain Marc17
Boudvillain M.16
Leng Marc13
Schwartz A.12
Margeat Emmanuel9
Rabhi Makhlouf7
Nadiras C.6
Leng M.6
Rahmouni A. Rachid6
Dalbies Rozenn5
Simon Isabelle5
Eveno E.5
Figueroa-Bossi N.4
Figueroa-Bossi Nara4
Bossi Lionello4
Nadiras Cédric3
Eveno Eric3
Margeat E.3
Malinge Jean-Marc3
Delaleau Mildred3
Nollmann Marcelo3
Bossi L.3
Lippert Bernhard2
Coste F.2
Castaing B.2
Rahmouni A.R.2
Walmacq C.2
Dalbies-Tran Rozenn2
Saridakis Emmanuel2
Vishwakarma Rishi2
Malinge Jean-Marc2
Walmacq Céline2
Rahmouni A. Rachid2
Coste Franck2
Jacquinot Frederique2
d'Heygere F.2
Bron Patrick2
Lavery R.1
Oteyza D.1
Bernal-Méndez Eloy1
Schmidt Kathrin1
van der Marel Gijs1
van Boom Jacques1
González-Vílchez Francisco1
Drumm Markus1
Sletten Einar1
Aussourd Christophe1
Porrua Odil1
Waldsich Christina1
M. Leng1
Montero Eva1
Fuertes Miguel1
Navarro-Ranninger Carmen1
Lemaire Marie-Agnes1
Ratel Mathilde1
Kerboriou Patricia1
Camilli Andrew1
Figueroa-Bossi Nara1
Decoville M.1
Locker D.1
Fedorova O.1
Kawaola J.1
Pyle A.M.1
Laurent Camille1
Gocheva V.1
d'Heygère F.1
Guillemardet B.1
Simon I.1
Soares Emilie1
Delaleau M.1
Manier O.1
Fiche J-B.1
Figueroa-Bosssi N.1
Vishwakarma R.1
Saridakis Emmanuel1
Nord A.1
Cohen-Gonsaud Martin1
Coste Franck1
Castaing Bertrand1
Delaleau Mildred1
Libri Domenico1
Martin Kevin1
Boudvillain Marc1
Lévêque Gaëtan1
Saison-Behmoaras Tula1
Reedijk Jan1
Gaucheron Frederic1
Prevost C.1
Colmenero J.1
Clerte C.1
Espéli Olivier1
Ramonda M.1
Bouloc Philippe1
Le Hir Hervé1
Rahmouni a Rachid1
Lemaire M.1
Cohen-Gonsaud Martin1
Lai-Kee-Him Josephine1
Revues
- Nucleic Acids Research9
- Biochemistry6
- Journal of Molecular Biology3
- Journal of Biomolecular Structure and Dynamics2
- EMBO Journal2
- Genes and Development2
- Proceedings of the National Academy of Sciences of the United States of America2
- Biochimica et Biophysica Acta - Gene Regulatory Mechanisms1
- Antisense and Nucleic Acid Drug Development1
- ChemBioChem1
- Chemistry - A European Journal1
- Current Opinion in Microbiology1
- Communications Biology1
- FEBS Letters1
- Trends in Genetics1
- Journal of Biological Inorganic Chemistry1
- Le Studium Multidisciplinary Journal1
- Methods in Enzymology1
- Nature Structural and Molecular Biology1
- PLoS Genetics1
- Metal Ions in Biological Systems1
- Bulletin du Cancer1
- Metal-Based Drugs1
- RNA Biology1
- Journal of Applied Physics1
- Journal of Biological Chemistry1
- Transcription1
- Microbiology1
Année de production
Institutions
- Université d'Orléans70
- Institut National de la Santé et de la Recherche Médicale70
- Institut de Chimie du CNRS70
- Centre National de la Recherche Scientifique70
- Université de Montpellier9
- Commissariat à l'énergie atomique et aux énergies alternatives5
- Université Paris-Saclay4
- Université Paris-Sud - Paris 114
- National Center for Scientific Research "Demokritos"2
- Université Claude Bernard Lyon 12
- Le Studium Loire Valley Institute for Advanced Studies, 45000 Orléans, France1
- Universiteit Leiden [Leiden]1
- University of Leeds1
- University of Alabama at Birmingham [ Birmingham]1
- Bureau de Recherches Géologiques et Minières (BRGM)1
- Aix Marseille Université1
- Institut Français du Cheval et de l'Equitation [Saumur]1
- Université Toulouse III - Paul Sabatier1
- Centre national du machinisme agricole, du génie rural, des eaux et forêts1
- Université de Tours1
- Université Paris Diderot - Paris 71
- Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail1
- Facultad de Ciencias, Universidad Autonoma de Madrid1
- Institut de recherche pour le développement [IRD] : UR1
- Frederick National Laboratory for Cancer Research1
- Institut National Polytechnique (Toulouse)1
- Materials Physics Center CSIC-UPV|EHU and Donostia International Physics Center, San Sebastián, Spain1
- Tufts University [Boston, MA, USA]1
- Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement1
- Muséum national d'Histoire naturelle1
- Institut National de la Recherche Agronomique1
- Université Pierre et Marie Curie - Paris 61
Laboratoires
- Centre de biophysique moléculaire70
- Centre de Biochimie Structurale [Montpellier]8
- Centre de génétique moléculaire4
- Institut de Biologie Intégrative de la Cellule4
- Institut de biologie et chimie des protéines [Lyon]2
- LE STUDIUM1
- Institut Jacques Monod1
- The Astbury Centre for Structural Molecular Biology1
- Physiologie de la reproduction et des comportements [Nouzilly]1
- Immunologie et Embryologie Moléculaires1
- Leiden Institute of Chemistry , Gorlaeus Laboratories1
- Structure et Instabilité des Génomes1
- Institut d’Electronique et des Systèmes1
- Centre de génétique moléculaire1
- Laboratoire de chimie bactérienne1
- Materials Physics Center CSIC-UPV|EHU and Donostia International Physics Center, San Sebastián, Spain1
- Department of Molecular Biology and Microbiology [Boston, MA, USA]1
- Institut de mécanique des fluides de Toulouse1
Départements
Équipes de recherche
Article dans une revue47 documents
- Emmanuel Saridakis, Rishi Vishwakarma, Josephine Lai-Kee-Him, Kevin Martin, Isabelle Simon, et al.. Cryo-EM structure of transcription termination factor Rho from Mycobacterium tuberculosis reveals bicyclomycin resistance mechanism. Communications Biology, Nature Publishing Group, 2022, 5 (1), pp.120. ⟨10.1038/s42003-022-03069-6⟩. ⟨hal-03563179⟩
- Isabelle Simon, Mildred Delaleau, Annie Schwartz, Marc Boudvillain. A large insertion domain in the Rho factor from a low G+C, Gram-negative bacterium is critical for RNA binding and transcription termination activity. Journal of Molecular Biology, Elsevier, 2021, 433 (15), pp.167060. ⟨10.1016/j.jmb.2021.167060⟩. ⟨hal-03248336⟩
- Lionello Bossi, Nara Figueroa-Bossi, Philippe Bouloc, Marc Boudvillain. Regulatory interplay between small RNAs and transcription termination factor Rho. Biochimica et Biophysica Acta - Gene Regulatory Mechanisms , Elsevier, 2020, pp.194546. ⟨10.1016/j.bbagrm.2020.194546⟩. ⟨hal-02533337⟩
- Lionello Bossi, Mathilde Ratel, Camille Laurent, Patricia Kerboriou, Andrew Camilli, et al.. NusG prevents transcriptional invasion of H-NS-silenced genes. PLoS Genetics, Public Library of Science, 2019, 15 (10), pp.e1008425. ⟨10.1371/journal.pgen.1008425⟩. ⟨hal-02308418⟩
- Cédric Nadiras, Mildred Delaleau, Annie Schwartz, Emmanuel Margeat, Marc Boudvillain. A Fluorogenic Assay To Monitor Rho-Dependent Termination of Transcription. Biochemistry, American Chemical Society, 2019, 58 (7), pp.865-874. ⟨10.1021/acs.biochem.8b01216⟩. ⟨hal-02067416⟩
- Cédric Nadiras, Eric Eveno, Annie Schwartz, Nara Figueroa-Bossi, Marc Boudvillain. A multivariate prediction model for Rho-dependent termination of transcription. Nucleic Acids Research, Oxford University Press, 2018, 46 (16), pp.8245 - 8260. ⟨10.1093/nar/gky563⟩. ⟨hal-01880575⟩
- Emmanuel Saridakis, Franck Coste, Bertrand Castaing, Marc Boudvillain. New crystallization strategies to fast-feed structure-guided pharmacological development -Applications to large biological assemblies involved in RNA metabolism. Le Studium Multidisciplinary Journal, Le Studium Multidisciplinary Journal, 2018, ⟨10.34846/Le-Studium.152.02.FR.01-2018⟩. ⟨hal-03542850⟩
- Odil Porrua, Marc Boudvillain, Domenico Libri. Transcription Termination: Variations on Common Themes. Trends in Genetics, Elsevier, 2016, 32 (8), pp.508 - 522. ⟨10.1016/j.tig.2016.05.007⟩. ⟨hal-01407669⟩
- F. d'Heygere, A. Schwartz, F. Coste, B. Castaing, M. Boudvillain. ATP-dependent motor activity of the transcription termination factor Rho from Mycobacterium tuberculosis. Nucleic Acids Research, Oxford University Press, 2015, 43, pp.6099-60111. ⟨10.1093/nar/gkv505⟩. ⟨hal-01171219⟩
- V. Gocheva, Antoine Le Gall, M. Boudvillain, E. Margeat, Marcelo Nollmann. Direct observation of the translocation mechanism of transcription termination factor Rho. Nucleic Acids Research, Oxford University Press, 2015, 43 (4), pp.2367-2377. ⟨10.1093/nar/gkv085⟩. ⟨hal-01171254⟩
- N. Figueroa-Bossi, A. Schwartz, B. Guillemardet, F. d'Heygere, L. Bossi, et al.. RNA remodeling by bacterial global regulator CsrA promotes Rho-dependent transcription termination. Genes and Development, Cold Spring Harbor Laboratory Press, 2014, 28 (11), pp.1239-1251. ⟨10.1101/gad.240192.114⟩. ⟨hal-01178971⟩
- Emilie Soares, Annie Schwartz, Marcelo Nollmann, Emmanuel Margeat, Marc Boudvillain. The RNA-mediated, asymmetric ring regulatory mechanism of the transcription termination Rho helicase decrypted by time-resolved nucleotide analog interference probing (trNAIP).. Nucleic Acids Research, Oxford University Press, 2014, 42 (14), pp.9270-9284. ⟨10.1093/nar/gku595⟩. ⟨hal-01180888⟩
- Francesca Fiorini, Marc Boudvillain, Hervé Le Hir. Tight intramolecular regulation of the human Upf1 helicase by its N- and C-terminal domains. Nucleic Acids Research, Oxford University Press, 2013, 41 (4), pp.2404-2415. ⟨10.1093/nar/gks1320⟩. ⟨hal-02358926⟩
- Rachid Menouni, Stéphanie Champ, Leon Espinosa, Marc Boudvillain, Mireille Ansaldi. Transcription termination controls prophage maintenance in Escherichia coli genomes.. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2013, 110 (35), pp.14414-9. ⟨10.1073/pnas.1303400110⟩. ⟨hal-00874882⟩
- Marc Boudvillain, Nara Figueroa-Bossi, Lionello Bossi. Terminator still moving forward: expanding roles for Rho factor.. Current Opinion in Microbiology, Elsevier, 2013, 16 (2), pp.118-24. ⟨10.1016/j.mib.2012.12.003⟩. ⟨hal-00840158⟩
- François d'Heygère, Makhlouf Rabhi, Marc Boudvillain. Phyletic distribution and conservation of the bacterial transcription termination factor Rho.. Microbiology, Microbiology Society, 2013, epub ahead of print. ⟨10.1099/mic.0.067462-0⟩. ⟨hal-00840167⟩
- Annie Schwartz, Makhlouf Rabhi, Emmanuel Margeat, Marc Boudvillain. Analysis of helicase-RNA interactions using nucleotide analog interference mapping.. Methods in Enzymology, Elsevier, 2012, 511, pp.149-69. ⟨10.1016/B978-0-12-396546-2.00007-3⟩. ⟨hal-00726256⟩
- Lionello Bossi, Annie Schwartz, Benoit Guillemardet, Marc Boudvillain, Nara Figueroa-Bossi. A role for Rho-dependent polarity in gene regulation by a noncoding small RNA.. Genes and Development, Cold Spring Harbor Laboratory Press, 2012, 26 (16), pp.1864-1873. ⟨10.1101/gad.195412.112⟩. ⟨hal-00727260⟩
- Makhlouf Rabhi, Veronika Gocheva, Frederique Jacquinot, Andrea Lee, Emmanuel Margeat, et al.. Mutagenesis-Based Evidence for an Asymmetric Configuration of the Ring-Shaped Transcription Termination Factor Rho. Journal of Molecular Biology, Elsevier, 2011, 405 (2), pp.497-518. ⟨10.1016/j.jmb.2010.11.006⟩. ⟨hal-00608241⟩
- Makhlouf Rabhi, Olivier Espéli, Annie Schwartz, Bastien Cayrol, a Rachid Rahmouni, et al.. The Sm-like RNA chaperone Hfq mediates transcription antitermination at Rho-dependent terminators.. EMBO Journal, EMBO Press, 2011, 30 (14), pp.2805-16. ⟨10.1038/emboj.2011.192⟩. ⟨hal-00720713⟩
- Makhlouf Rabhi, Roman Tuma, Marc Boudvillain. RNA remodeling by hexameric RNA helicases. RNA Biology, Taylor & Francis, 2010, 7 (6 - Sp. Iss. SI), pp.655-666. ⟨10.4161/rna.7.6.13570⟩. ⟨hal-00602496⟩
- Marc Boudvillain, Marcelo Nollmann, Emmanuel Margeat. Keeping up to speed with the transcription termination factor Rho motor.. Transcription, Taylor & Francis, 2010, 1 (2), pp.70-75. ⟨10.4161/trns.1.2.12232⟩. ⟨hal-00591655⟩
- C. Riedel, R. Arinero, Philippe Tordjeman, M. Ramonda, Gaëtan Lévêque, et al.. Determination of the nanoscale dielectric constant by means of a double pass method using electrostatic force microscopy. Journal of Applied Physics, American Institute of Physics, 2009, 106 (2), ⟨10.1063/1.3182726⟩. ⟨hal-01791002⟩
- Annie Schwartz, Makhlouf Rabhi, Frederique Jacquinot, Emmanuel Margeat, A. Rachid Rahmouni, et al.. A stepwise 2 '-hydroxyl activation mechanism for the bacterial transcription termination factor Rho helicase. Nature Structural and Molecular Biology, Nature Publishing Group, 2009, 16 (12), pp.1309-1316. ⟨10.1038/nsmb.1711⟩. ⟨hal-00525325⟩
- Annie Schwartz, Céline Walmacq, A. Rachid Rahmouni, Marc Boudvillain. Noncanonical Interactions in the Management of RNA Structural Blocks by the Transcription Termination Rho Helicase. Biochemistry, American Chemical Society, 2007, 46 (33), pp.9366 - 9379. ⟨10.1021/bi700493m⟩. ⟨hal-00166551⟩
- Annie Schwartz, Emmanuel Margeat, A. Rachid Rahmouni, Marc Boudvillain. Transcription termination factor Rho can displace streptavidin from biotinylated RNA. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2007, 282 (43), pp.31469-31476. ⟨10.1074/jbc.M706935200⟩. ⟨hal-00176284⟩
- C. Walmacq, A.R. Rahmouni, M. Boudvillain. Testing the steric exclusion model for hexameric helicases: substrate features that alter RNA-DNA unwinding by the transcription termination factor Rho. Biochemistry, American Chemical Society, 2006, 45, pp.5885-5895. ⟨10.1021/bi0600648⟩. ⟨hal-00088941⟩
- C. Walmacq, A.R. Rahmouni, M. Boudvillain. Influence of substrate composition on the helicase activity of transcription termination factor Rho: reduced processivity of Rho hexamers during unwinding of RNA-DNA hybrid regions. Journal of Molecular Biology, Elsevier, 2004, 342 (2), pp.403-420. ⟨10.1016/j.jmb.2004.07.026⟩. ⟨hal-00113090⟩
- A. Schwartz, A. Rachid Rahmouni, Marc Boudvillain. The functional anatomy of an intrinsic transcription terminator. EMBO Journal, EMBO Press, 2003, 22 (13), pp.3385 - 3394. ⟨10.1093/emboj/cdg310⟩. ⟨hal-01880611⟩
- Marc Boudvillain, Annie Schwartz, A. Rachid Rahmouni. Limited Topological Alteration of the T7 RNA Polymerase Active Center at Intrinsic Termination Sites †. Biochemistry, American Chemical Society, 2002, 41 (9), pp.3137-3146. ⟨10.1021/bi011358q⟩. ⟨hal-02075291⟩
- Kathrin Schmidt, Marc Boudvillain, Annie Schwartz, Gijs van der Marel, Jacques van Boom, et al.. Monofunctionallytrans-Diammine Platinum(II)-Modified Peptide Nucleic Acid Oligomers: A New Generation of Potential Antisense Drugs. Chemistry - A European Journal, Wiley-VCH Verlag, 2002, 8 (24), pp.5566 - 5570. ⟨10.1002/1521-3765(20021216)8:24<5566::AID-CHEM5566>3.0.CO;2-#⟩. ⟨hal-01880614⟩
- Eva Montero, José Pérez, Annie Schwartz, Miguel Fuertes, Jean-Marc Malinge, et al.. Apoptosis induction and DNA interstrand cross-link formation by cytotoxic trans-[PtCl2(NH(CH3)2)(NHCH(CH3)2) : cross-linking between d(G) and complementary d(C) within oligonucleotide duplexes.. ChemBioChem, Wiley-VCH Verlag, 2002, 3 (1), pp.61-7. ⟨hal-02132063⟩
- Boudvillain Marc, Rozenn Dalbies-Tran, Marc Leng, Marc Boudvillain. Oligonucleotides Modified With Transplatin Derivatives: Fast and Efficient Metalloribozymes. Metal-Based Drugs, Hindawi Publishing Corporation, 2001, 8 (1), pp.39 - 45. ⟨10.1155/MBD.2001.39⟩. ⟨hal-01880601⟩
- Jens Müller, Markus Drumm, Marc Boudvillain, Marc Leng, Einar Sletten, et al.. Parallel-stranded DNA with Hoogsteen base pairing stabilized by a trans-[Pt(NH3)2]2+ cross-link: characterization and conversion into a homodimer and a triplex. Journal of Biological Inorganic Chemistry, Springer Verlag, 2000, 5 (5), pp.603-611. ⟨10.1007/s007750000143⟩. ⟨hal-02075297⟩
- Françoise Paquet, Marc Boudvillain, Gérard Lancelot, Marc Leng. NMR solution structure of a DNA dodecamer containing a transplatin interstrand GN7-CN3 cross-link. Nucleic Acids Research, Oxford University Press, 1999, 27 (21), pp.4261-4268. ⟨10.1093/nar/27.21.4261⟩. ⟨hal-02075311⟩
- Caroline Colombier, Marc Boudvillain, Marc Leng. Interstrand Crosslinking Reaction in Transplatin-Modified Oligo-2‵-O-Methyl Ribonucleotide-RNA Hybrids. Antisense and Nucleic Acid Drug Development, Mary Ann Liebert, 1997, 7 (4), pp.397-402. ⟨10.1089/oli.1.1997.7.397⟩. ⟨hal-02322135⟩
- Eloy Bernal-Méndez, Marc Boudvillain, Francisco González-Vílchez, Marc Leng. Chemical Versatility of Transplatin Monofunctional Adducts within Multiple Site-Specifically Platinated DNA †. Biochemistry, American Chemical Society, 1997, 36 (24), pp.7281 - 7287. ⟨10.1021/bi9703148⟩. ⟨hal-01880622⟩
- Marc Boudvillain, Martine Guerin, Rozenn Dalbies, Tula Saison-Behmoaras, Marc Leng. Transplatin-Modified Oligo(2‘- O -methyl ribonucleotide)s: A New Tool for Selective Modulation of Gene Expression †. Biochemistry, American Chemical Society, 1997, 36 (10), pp.2925 - 2931. ⟨10.1021/bi962695f⟩. ⟨hal-01880625⟩
- Marc Boudvillain, C. Prevost, P. Beudaert, M. Leng, R. Lavery, et al.. Distortions of the DNA Double Helix Induced by 1,3-trans-Diamminedichloroplatinum(II)-intrastrand Cross-link: An Internal Coordinate Molecular Modeling Study. Journal of Biomolecular Structure and Dynamics, Taylor & Francis: STM, Behavioural Science and Public Health Titles, 1997, 14 (6), pp.703 - 714. ⟨10.1080/07391102.1997.10508173⟩. ⟨hal-01880624⟩
- Marc Boudvillain, Rozenn Dalbies, M. Leng. Evidences for a catalytic activity of the DNA double helix in the reaction between DNA, platinum(II), and intercalators.. Metal Ions in Biological Systems, Marcel Dekker, 1996, 33, pp.87-104. ⟨hal-02322086⟩
- Marc Boudvillain, Rozenn Dalbies, Christophe Aussourd, Marc Leng. Intrastrand cross-links are not formed in the reaction between transplatin and native DNA: relation with the clinical inefficency of transplatin. Nucleic Acids Research, Oxford University Press, 1995, 23 (13), pp.2381-2388. ⟨10.1093/nar/23.13.2381⟩. ⟨hal-02075318⟩
- Rozenn Dalbies, Marc Boudvillain, Marc Leng. Linkage isomerization reaction of intrastrand cross-links in trans -diamminedichloroplatinum(II)-modified single-stranded oligonucleotides. Nucleic Acids Research, Oxford University Press, 1995, 23 (6), pp.949-953. ⟨10.1093/nar/23.6.949⟩. ⟨hal-02075321⟩
- M. Decoville, A. Schwartz, D. Locker, M. Leng. Detection of minor adducts in cisplatin-modified DNA by transcription footprinting.. FEBS Letters, Wiley, 1993, 323 (1-2), pp.55-8. ⟨hal-02119335⟩
- M. Lemaire, A. Rachid Rahmouni, A. Schwartz, M. Leng. Interstrand cross-links are preferentially formed at the d(GC) sites in the reaction between cis-diamminedichloroplatinum (II) and DNA.. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 1991, 88 (5), pp.1982-1985. ⟨10.1073/pnas.88.5.1982⟩. ⟨hal-02127202⟩
- Jean-Marc Malinge, Annie Schwartz, M. Leng. Reaction of nucleic-acids with cis-dichlorodiammineplatinum (II) in the presence of intercalating agents - characterization of new ternary complexes. Bulletin du Cancer, John Libbey Eurotext, 1988, 75 (7), pp.668. ⟨hal-02171328⟩
- Jean-Marc Malinge, Annie Schwartz, Marc Leng. Characterization of the ternary complexes formed in the reaction of cis-diamminedichloro platinum (II), ethidium bromide and nucleic acids. Nucleic Acids Research, Oxford University Press, 1987, 15 (4), pp.1779-1797. ⟨10.1093/nar/15.4.1779⟩. ⟨hal-02134724⟩
- A. Rachid Rahmouni, Jean-Marc Malinge, Annie Schwartz, Marc Leng. Comparison Between poly(dG-dC)·poly(dG-dC) and DNA Modified by cis-diamminedichloroplatinum (II): Immunological and Spectroscopic Studies. Journal of Biomolecular Structure and Dynamics, Taylor & Francis: STM, Behavioural Science and Public Health Titles, 1985, 3 (2), pp.363-375. ⟨10.1080/07391102.1985.10508423⟩. ⟨hal-02127084⟩
Communication dans un congrès1 document
- Emmanuel Saridakis, Rishi Vishwakarma, Joséphine Lai Kee Him, Kevin Martin, Isabelle Simon, et al.. Structural basis for resistance of Mycobacterium tuberculosis Rho to bicyclomycin. Helicases & Nucleic Acid-Based Machines: Structure, Mechanism & Regulation, and Roles in HumanDisease, Jul 2021, Paris, France. ⟨hal-03419029⟩
Poster10 documents
- Isabelle Simon, Mildred Delaleau, Annie Schwartz, Marc Boudvillain. Variation on a theme - the unusual features of the essential transcription termination Rho factor from a low G+C, Gram-negative bacterium. 45th FEBS congress, Jul 2021, Lubjana, Slovenia. ⟨hal-03419044⟩
- E. Eveno, C. Nadiras, N. Figueroa-Bossi, A. Schwartz, M. Boudvillain. Predicting Rho-dependent termination of transcription at genome scale. 24th Annual Meeting of the RNA Society, Jun 2019, Cracovie, Poland. ⟨hal-02902104⟩
- I. Simon, A. Schwartz, M. Delaleau, L. Bossi, N. Figueroa-Bossi, et al.. Exploring Rho helicase activity for the purpose of synthetic regulation. 32e Colloque Biotechnocentre, Oct 2019, Seillac, France. ⟨hal-02902096⟩
- E. Eveno, C. Nadiras, A. Schwartz, N. Figueroa-Bossi, M. Boudvillain. Genome-scale prediction of Rho-dependent termination of transcription. 11e Rencontre SifrARN (SFBBM), Nov 2018, Nancy, France. ⟨hal-02902107⟩
- C. Nadiras, E. Eveno, M. Boudvillain. Seeking Rho-dependent transcription termination sites within bacterial genomes. EMBO & Harden conference - Helicases and Nucleic Acid Translocases, Jul 2017, Kloster Banz-Bamberg, Germany. ⟨hal-02902120⟩
- C. Nadiras, E. Eveno, M. Boudvillain. Towards a model for predicting Rho-dependent transcription termination sites. Le Studium conference “Structural biology and biophysics of RNA-protein complexes”, Nov 2017, Orléans, France. ⟨hal-02902112⟩
- C. Nadiras, E. Eveno, M. Boudvillain. Identification des sites de terminaison de transcription Rho-dépendantes dans les génomes bactériens. 30e Colloque Biotechnocentre, Oct 2017, Seillac, France. ⟨hal-02902116⟩
- C. Clerte, O. Manier, J-B. Fiche, M. Boudvillain, L. Bossi, et al.. Relationships between Rho and RNA polymerase in living bacteria. Le Studium conference “Structural biology and biophysics of RNA-protein complexes”, Nov 2017, Orléans, France. ⟨hal-02902111⟩
- R. Vishwakarma, A. Nord, F. Pedaci, M. Boudvillain, E. Margeat. Single-molecule analysis of E. coli Rho translocase, Helicase, transcription termination activities, and its interactions with RNA polymerase. Le Studium conference “Structural biology and biophysics of RNA-protein complexes”, Nov 2017, Orléans, France. ⟨hal-02902115⟩
- C. Nadiras, Eric Eveno, A. Schwartz, M. Boudvillain. Analyse des terminateurs Rho-dépendants bactériens. 29e colloque Biotechnocentre, Oct 2016, Seillac, France. ⟨hal-02453368⟩
Ouvrage (y compris édition critique et traduction)1 document
- Marc Boudvillain. Methods in Molecular Biology - RNA remodeling proteins. 2021, 978-1-0716-0935-4. ⟨10.1007/978-1-0716-0935-4⟩. ⟨hal-03036533⟩
Chapitre d'ouvrage10 documents
- Isabelle Simon, Marc Boudvillain. A simple fluorescence microplate assay to monitor RNA-DNA hybrid unwinding by the bacterial transcription termination factor Rho. Marc Boudvillain. RNA remodeling proteins, 2209, Humana press, pp.143-161, 2021, Methods in Molecular Biology, ⟨10.1007/978-1-0716-0935-4_10⟩. ⟨hal-03036494⟩
- Cédric Nadiras, Annie Schwartz, Mildred Delaleau, Marc Boudvillain. Evaluating the Effect of Small RNAs and Associated Chaperones on Rho-Dependent Termination of Transcription In Vitro.. Methods in Mecular Biology, 1737, humana press, Totowa, NJ-USA, pp.99-118, 2018, Bacterial regulatory RNA (Arluison & Valverde, eds), ⟨10.1007/978-1-4939-7634-8_7⟩. ⟨hal-01880631⟩
- F. d'Heygère, A. Schwartz, F. Coste, B. Castaing, M. Boudvillain. Monitoring RNA Unwinding by the Transcription Termination Factor Rho from Mycobacterium tuberculosis. Methods in Mecular Biology, 1259, Springer, pp.293-311, 2015, RNA remodelling proteins (Boudvillain, ed), 978-1-4939-2213-0. ⟨10.1007/978-1-4939-2214-7_18⟩. ⟨hal-01174055⟩
- Olga Fedorova, Marc Boudvillain, Christina Waldsich. Nucleotide Analog Interference Mapping and Suppression (NAIM/NAIS): a Combinatorial Approach to Study RNA Structure, Folding, and Interaction with Proteins. Handbook of RNA Biochemistry, 2nd edition (Westhof, Bindereif, Schon & Hartmann, eds), Wiley-VCH Verlag GmbH & Co. KGaA, pp.329-368, 2014, ⟨10.1002/9783527647064.ch17⟩. ⟨hal-02097735⟩
- Makhlouf Rabhi, Rahmouni Rachid A., Marc Boudvillain. Transcription termination factor Rho: a ring-shaped RNA helicase from bacteria. Eckhard Jankowsky. RNA helicases, Royal Society of Chemistry Publishing, pp.243-271, 2010, ⟨10.1039/9781849732215-00243⟩. ⟨hal-00610004⟩
- Marc Boudvillain, Céline Walmacq, Annie Schwartz, Frédérique Jacquinot. Simple enzymatic assays for the in vitro motor activity of transcription termination factor Rho from Escherichia coli. Mohamed M. M. Abdelhaleem. Methods in Mecular Biology, Humana Press, pp.137-154, 2009, Helicases (Abdelhaleem, ed), ⟨10.1007/978-1-60327-355-8_10⟩. ⟨hal-00623571⟩
- O. Fedorova, M. Boudvillain, J. Kawaola, A.M. Pyle. Nucleotide analog interference mapping and suppression (NAIM/NAIS): specific applications in studies of RNA tertiary structure, dynamic helicase mechanism, and RNA-protrein interactions. E. Westhof, A. Bindereif, A. Schon, R.I. Hartmann. Handbook of RNA biochemistry, Wiley-VCH, pp.259-293, 2005. ⟨hal-00088591⟩
- Marc Boudvillain, Rozenn Dalbies-Tran, Leng M.. Trans-Diamminedichloroplatinum (II) Is Not an Antitumor Drug: Why?. Platinum and Other Metal Coordination Compounds in Cancer Chemotherapy, 2, Plenum Press, New'Tork, USA, pp.17-25, 1996. ⟨hal-02097755⟩
- Frederic Gaucheron, Marie-Agnes Lemaire, Jean-Marc Malinge, Annie Schwartz, Marc Leng. Reactivity of DNA and cis-Diamminedichloroplatinumul(II) in the Presence of Intercalating Agents. Platinum and Other Metal Coordination Compounds in Cancer Chemotherapy, Springer US, pp.73-79, 1991, 978-1-4899-0740-0. ⟨hal-02134699⟩
- A. Rachid Rahmouni, A. Schwartz, M. Leng. Importance of DNA Sequence in the Reaction of d(ApG) and d(GpA) with Cis-Diamminedichloroplatinum (II). Platinum and Other Metal Coordination Compounds in Cancer Chemotherapy: Proceedings of the Fifth International Symposium on Platinum and Other Metal Coordination Compounds in Cancer Chemotherapy Abano, Padua, ITALY - June 29–July 2, 1987, Springer US, pp.127-131, 1988. ⟨hal-02127164⟩
Brevet1 document
- Marc Leng, Rozenn Dalbies, Marc Boudvillain. Procédé de pontage sélectif et irréversible d'oligonucléotides platinés sur de l'ARN et ses applications. France, Patent n° : WO1998018915A2. 1996. ⟨hal-02097839⟩