This group investigates structure-function relationships in RNA-based biological systems. Research is focused on RNA remodelling proteins (helicases, chaperones). The group notably develops sophisticated biochemical and bioinformatics approaches to decrypt the molecular mechanisms of these essential factors.
Research theme
The group explores the mechanisms by which the structures of RNA molecules and ribonucleoproteic (RNP) complexes are remodelled. These structures are often dynamic and can evolve within the cell where they govern the role and fate of RNA species. Two main classes of ubiquitous proteins –RNA chaperones and RNA helicases- shape and remodel the structures of RNA and RNP complexes. The dysfunction of endogenous RNA remodelling proteins or the action of viral or bacterial ones are implicated in diverse pathologies including cancers. We study how RNA helicases and RNA chaperones work and cooperate to drive key physiological functions.
RNA chperones usually disrupt secondary structures upon their binding to RNA and promote the formation of alternative structural motifs and/or complexes. RNA helicases use the energy derived from their NTPase activity to disrupt double-stranded RNA, RNA-DNA heteroduplexes, or RNP complexes. These activities are sometimes linked to directional translocation of the helicase along RNA.
To study RNA remodelling proteins, we use complementary biological, biochemical, and biophysical approaches. We focus our efforts on a complex model system wherein Rho, a ring-shaped RNA helicase, is involved in transcription termination and genome maintenance in bacteria.
We study the molecular mechanisms governing Rho activity, how the helicase is involved in gene silencing mechanisms implicating non-coding RNAs and RNA chaperones (Hfq, CsrA), the importance of Rho for bacterial pathogenicity and fitness, how the functions and modes of action of Rho are conserved within the biodiversity, etc.
We are also investigating other, distinct RNA helicase specimens. In this way, we hope to understand the basic mechanisms that govern these important enzymes, determine their key common features and differences, and identify factors that contribute to their regulation.
Our long-term goal is to exploit this information for biomedical purposes and for the design of biosensors or inducible gene circuits useful for synthetic biology.
Significant publications :
- Cédric Nadiras, Eric Eveno, Annie Schwartz, Nara Figueroa-Bossi, Marc Boudvillain.
A multivariate prediction model for Rho-dependent termination of transcription.
Nucleic Acids Research (2018) 46, 8245-8260 - Odil Porrua, Marc Boudvillain, Domenico Libri.
Transcription Termination: Variations on Common Themes.
Trends in Genetics (2016) 32, 508-522. - Emilie Soares, Annie Schwartz, Marcello Nollmann, Emmanuel Margeat, Marc Boudvillain.
The RNA-mediated, asymmetric ring regulatory mechanism of the transcription termination Rho helicase decrypted by time-resolved nucleotide analog interference probing (trNAIP).
Nucleic Acids Research (2014) 9270-9284. - Nara Figueroa-Bossi, Annie Schwartz, Benoit Gillemardet, François D’Heygère, Lionello Bossi, Marc Boudvillain
RNA remodeling by bacterial global regulator CsrA promotes Rho-dependent transcription termination
Genes & Development (2014) 28, 1239-51. - Rachid Menouni, Stéphanie Champ, Léon Espinosa, Marc Boudvillain, Mireille Ansaldi
Transcription termination controls prophage maintenance in Escherichia coli genomes
Proc. Natl. Acad. Sci USA (2013) 110, 14414-14419.
- Filtres
Publications (Archives Hal)
Contact
Disciplines
- Sciences du Vivant [q-bio]34
- Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]11
- Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]/Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire11
- Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]/Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire/Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie structurale [q-bio.BM]10
- Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire7
- Sciences du Vivant [q-bio]/Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire5
- Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie structurale [q-bio.BM]/Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire/Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]4
- Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie structurale [q-bio.BM]/Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire4
- Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie structurale [q-bio.BM]4
- Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire/Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]1
- Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire1
- Sciences de l'ingénieur [physics]1
Mots-clefs
- Rho5
- Helicase4
- Transcription termination4
- ESCHERICHIA-COLI RHO3
- Hexamer3
- RNA3
- Molecular motor2
- STRUCTURAL BASIS2
- Termination2
- Transcription2
- ACTIVATION1
- ADDUCTS1
- APOPTOSIS1
- ATP-BINDING DOMAIN1
- ATPASE ACTIVATION1
- BACTERIOPHAGE PHI-61
- BINDING1
- CIS1
- CIS-DIAMMINEDICHLOROPLATINUMII1
- CISPLATIN1
- CONFORMATIONAL-CHANGES1
- CRO MESSENGER-RNA1
- ChiP1
- DEFECTIVE-MUTANTS1
- DEPENDENT TERMINATION1
- DNA1
- DNA HELICASE1
- DNAB HELICASE1
- DRUG1
- ELONGATION COMPLEX1
- FORM1
- Fluorescence1
- HEPATITIS-C VIRUS1
- HEXAMERIC HELICASE1
- HEXAMERIC HELICASES1
- Hexameric helicase1
- IDENTIFICATION1
- IN-VITRO1
- MOLECULAR MOTOR1
- Mechanism1
- Noncoding small RNA1
- NusG1
- P41
- PACKAGING MOTOR1
- PLATINUM ANTITUMOR COMPLEXES1
- POLYMERASE COMPLEX1
- PROTEIN1
- PROTEIN-RHO1
- Packaging1
- Processivity1
- RNA-BINDING DOMAIN1
- RNA-DNA HELICASE1
- Rho-dependent termination1
- Ring shaped1
- Rut1
- SECONDARY STRUCTURE1
- STABILITY1
- Translocation1
- UNZIPPING MECHANISM1
- Virus TRANSCRIPTION TERMINATION FACTOR1
Auteurs
Boudvillain Marc23
Schwartz Annie18
Boudvillain Marc18
Boudvillain M.16
Leng Marc13
Schwartz A.12
Margeat Emmanuel7
Rabhi Makhlouf7
Nadiras C.6
Leng M.6
Rahmouni A. Rachid6
Eveno E.5
Dalbiès Rozenn4
Figueroa-Bossi N.4
Figueroa-Bossi Nara4
Bossi Lionello4
Nadiras Cédric3
Eveno Eric3
Margeat E.3
Malinge Jean-Marc3
Nollmann Marcelo3
Bossi L.3
Lippert Bernhard2
Coste F.2
Castaing B.2
Rahmouni A.R.2
Walmacq C.2
Malinge Jean-Marc2
Walmacq Céline2
Rahmouni A. Rachid2
Le Hir Hervé2
Delaleau Mildred2
Jacquinot Frederique2
d'Heygere F.2
Fiorini Francesca2
Lavery R.1
Oteyza D.1
Bernal-Méndez Eloy1
Schmidt Kathrin1
van der Marel Gijs1
van Boom Jacques1
González-Vílchez Francisco1
Drumm Markus1
Sletten Einar1
Aussourd Christophe1
Porrua Odil1
Waldsich Christina1
R. Dalbiès1
M. Leng1
Montero Eva1
Fuertes Miguel1
Navarro-Ranninger Carmen1
Lemaire Marie-Agnes1
Ratel Mathilde1
Kerboriou Patricia1
Camilli Andrew1
Figueroa-Bossi Nara1
Dalbiès R1
Decoville M.1
Locker D.1
Fedorova O.1
Kawaola J.1
Pyle A.M.1
Laurent Camille1
Gocheva V.1
d'Heygère F.1
Guillemardet B.1
Simon I.1
Soares Emilie1
Delaleau M.1
Manier O.1
Fiche J-B.1
Figueroa-Bosssi N.1
Vishwakarma R.1
Simon Isabelle1
Nord A.1
Libri Domenico1
Saison-Behmoaras Tula1
Reedijk Jan1
Gaucheron Frederic1
Le Gall A.1
Prevost C.1
Colmenero J.1
Clerte C.1
Espéli Olivier1
Ramonda M.1
Bouloc Philippe1
Rahmouni a Rachid1
Lemaire M.1
Lévêque G.1
Beudaert P.1
Arinero R.1
Alonso Carlos1
Fedorova Olga1
Guerin Martine1
Müller Jens1
Riedel C.1
Tordjeman Ph.1
Lancelot Gérard1
Tuma Roman1
Revues
- Nucleic Acids Research10
- Biochemistry6
- Journal of Biomolecular Structure and Dynamics2
- EMBO Journal2
- Genes and Development2
- Journal of Molecular Biology2
- Proceedings of the National Academy of Sciences of the United States of America2
- Biochimica et Biophysica Acta - Gene Regulatory Mechanisms1
- Antisense and Nucleic Acid Drug Development1
- ChemBioChem1
- Chemistry - A European Journal1
- Current Opinion in Microbiology1
- FEBS Letters1
- Trends in Genetics1
- Journal of Biological Inorganic Chemistry1
- Methods in Enzymology1
- Nature Structural and Molecular Biology1
- PLoS Genetics1
- Metal Ions in Biological Systems1
- Bulletin du Cancer1
- Metal-Based Drugs1
- RNA Biology1
- Journal of Applied Physics1
- Journal of Biological Chemistry1
- Transcription1
- Microbiology1
Année de production
Institutions
- Université d'Orléans66
- Institut National de la Santé et de la Recherche Médicale66
- Centre National de la Recherche Scientifique66
- Université de Montpellier8
- Commissariat à l'énergie atomique et aux énergies alternatives4
- Université Paris-Saclay4
- Université Paris-Sud - Paris 114
- Université Claude Bernard Lyon 12
- Universiteit Leiden [Leiden]1
- University of Leeds1
- University of Alabama at Birmingham [ Birmingham]1
- Aix Marseille Université1
- Institut Français du Cheval et de l'Equitation [Saumur]1
- Université Toulouse III - Paul Sabatier1
- Institut National de Recherche en Informatique et en Automatique1
- Université de Tours1
- Université Paris Diderot - Paris 71
- Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail1
- Facultad de Ciencias, Universidad Autonoma de Madrid1
- Frederick National Laboratory for Cancer Research1
- Institut National Polytechnique (Toulouse)1
- Materials Physics Center CSIC-UPV|EHU and Donostia International Physics Center, San Sebastián, Spain1
- Tufts University [Boston, MA, USA]1
- Muséum national d'Histoire naturelle1
- Institut National de la Recherche Agronomique1
- Université Pierre et Marie Curie - Paris 61
Laboratoires
- Centre de biophysique moléculaire66
- Centre de Biochimie Structurale [Montpellier]7
- Centre de génétique moléculaire4
- Institut de Biologie Intégrative de la Cellule4
- Institut de biologie et chimie des protéines [Lyon]2
- Institut Jacques Monod1
- The Astbury Centre for Structural Molecular Biology1
- Physiologie de la reproduction et des comportements [Nouzilly]1
- Immunologie et embryologie moléculaires1
- Inria Siège1
- Leiden Institute of Chemistry , Gorlaeus Laboratories1
- Structure et Instabilité des Génomes1
- Institut d’Electronique et des Systèmes1
- Centre de génétique moléculaire1
- Laboratoire de chimie bactérienne1
- Materials Physics Center CSIC-UPV|EHU and Donostia International Physics Center, San Sebastián, Spain1
- Department of Molecular Biology and Microbiology [Boston, MA, USA]1
- Institut de mécanique des fluides de Toulouse1
Départements
Équipes de recherche
Article dans une revue45 documents
- Lionello Bossi, Nara Figueroa-Bossi, Philippe Bouloc, Marc Boudvillain. Regulatory interplay between small RNAs and transcription termination factor Rho. Biochimica et Biophysica Acta - Gene Regulatory Mechanisms , Elsevier, 2020, pp.194546. ⟨10.1016/j.bbagrm.2020.194546⟩. ⟨hal-02533337⟩
- Lionello Bossi, Mathilde Ratel, Camille Laurent, Patricia Kerboriou, Andrew Camilli, et al.. NusG prevents transcriptional invasion of H-NS-silenced genes. PLoS Genetics, Public Library of Science, 2019, 15 (10), pp.e1008425. ⟨10.1371/journal.pgen.1008425⟩. ⟨hal-02308418⟩
- Cédric Nadiras, Mildred Delaleau, Annie Schwartz, Emmanuel Margeat, Marc Boudvillain. A Fluorogenic Assay To Monitor Rho-Dependent Termination of Transcription. Biochemistry, American Chemical Society, 2019, 58 (7), pp.865-874. ⟨10.1021/acs.biochem.8b01216⟩. ⟨hal-02067416⟩
- Cédric Nadiras, Eric Eveno, Annie Schwartz, Nara Figueroa-Bossi, Marc Boudvillain. A multivariate prediction model for Rho-dependent termination of transcription. Nucleic Acids Research, Oxford University Press, 2018, 46 (16), pp.8245 - 8260. ⟨10.1093/nar/gky563⟩. ⟨hal-01880575⟩
- Odil Porrua, Marc Boudvillain, Domenico Libri. Transcription Termination: Variations on Common Themes. Trends in Genetics, Elsevier, 2016, 32 (8), pp.508 - 522. ⟨10.1016/j.tig.2016.05.007⟩. ⟨hal-01407669⟩
- V. Gocheva, A. Le Gall, M. Boudvillain, E. Margeat, Marcelo Nollmann. Direct observation of the translocation mechanism of transcription termination factor Rho. Nucleic Acids Research, Oxford University Press, 2015, 43 (4), pp.2367-2377. ⟨10.1093/nar/gkv085⟩. ⟨hal-01171254⟩
- F. d'Heygere, A. Schwartz, F. Coste, B. Castaing, M. Boudvillain. ATP-dependent motor activity of the transcription termination factor Rho from Mycobacterium tuberculosis. Nucleic Acids Research, Oxford University Press, 2015, 43, pp.6099-60111. ⟨10.1093/nar/gkv505⟩. ⟨hal-01171219⟩
- Emilie Soares, Annie Schwartz, Marcelo Nollmann, Emmanuel Margeat, Marc Boudvillain. The RNA-mediated, asymmetric ring regulatory mechanism of the transcription termination Rho helicase decrypted by time-resolved nucleotide analog interference probing (trNAIP).. Nucleic Acids Research, Oxford University Press, 2014, 42 (14), pp.9270-9284. ⟨10.1093/nar/gku595⟩. ⟨hal-01180888⟩
- N. Figueroa-Bossi, A. Schwartz, B. Guillemardet, F. d'Heygere, L. Bossi, et al.. RNA remodeling by bacterial global regulator CsrA promotes Rho-dependent transcription termination. Genes and Development, Cold Spring Harbor Laboratory Press, 2014, 28 (11), pp.1239-1251. ⟨10.1101/gad.240192.114⟩. ⟨hal-01178971⟩
- Marc Boudvillain, Nara Figueroa-Bossi, Lionello Bossi. Terminator still moving forward: expanding roles for Rho factor.. Current Opinion in Microbiology, Elsevier, 2013, 16 (2), pp.118-24. ⟨10.1016/j.mib.2012.12.003⟩. ⟨hal-00840158⟩
- François d'Heygère, Makhlouf Rabhi, Marc Boudvillain. Phyletic distribution and conservation of the bacterial transcription termination factor Rho.. Microbiology, Microbiology Society, 2013, epub ahead of print. ⟨10.1099/mic.0.067462-0⟩. ⟨hal-00840167⟩
- Francesca Fiorini, Marc Boudvillain, Hervé Le Hir. Tight intramolecular regulation of the human Upf1 helicase by its N- and C-terminal domains. Nucleic Acids Research, Oxford University Press, 2013, 41 (4), pp.2404-2415. ⟨10.1093/nar/gks1320⟩. ⟨hal-02358926⟩
- Francesca Fiorini, Marc Boudvillain, Hervé Le Hir. Tight intramolecular regulation of the human Upf1 helicase by its N- and C-terminal domains. Nucleic Acids Research, Oxford University Press, 2013, 41, pp.2404-2415. ⟨10.1093/nar/gks1320⟩. ⟨hal-00817448⟩
- Rachid Menouni, Stéphanie Champ, Leon Espinosa, Marc Boudvillain, Mireille Ansaldi. Transcription termination controls prophage maintenance in Escherichia coli genomes.. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2013, 110 (35), pp.14414-9. ⟨10.1073/pnas.1303400110⟩. ⟨hal-00874882⟩
- Annie Schwartz, Makhlouf Rabhi, Emmanuel Margeat, Marc Boudvillain. Analysis of helicase-RNA interactions using nucleotide analog interference mapping.. Methods in Enzymology, Elsevier, 2012, 511, pp.149-69. ⟨10.1016/B978-0-12-396546-2.00007-3⟩. ⟨hal-00726256⟩
- Lionello Bossi, Annie Schwartz, Benoit Guillemardet, Marc Boudvillain, Nara Figueroa-Bossi. A role for Rho-dependent polarity in gene regulation by a noncoding small RNA.. Genes and Development, Cold Spring Harbor Laboratory Press, 2012, 26 (16), pp.1864-1873. ⟨10.1101/gad.195412.112⟩. ⟨hal-00727260⟩
- Makhlouf Rabhi, Olivier Espéli, Annie Schwartz, Bastien Cayrol, a Rachid Rahmouni, et al.. The Sm-like RNA chaperone Hfq mediates transcription antitermination at Rho-dependent terminators.. EMBO Journal, EMBO Press, 2011, 30 (14), pp.2805-16. ⟨10.1038/emboj.2011.192⟩. ⟨hal-00720713⟩
- Makhlouf Rabhi, Veronika Gocheva, Frederique Jacquinot, Andrea Lee, Emmanuel Margeat, et al.. Mutagenesis-Based Evidence for an Asymmetric Configuration of the Ring-Shaped Transcription Termination Factor Rho. Journal of Molecular Biology, Elsevier, 2011, 405 (2), pp.497-518. ⟨10.1016/j.jmb.2010.11.006⟩. ⟨hal-00608241⟩
- Marc Boudvillain, Marcelo Nollmann, Emmanuel Margeat. Keeping up to speed with the transcription termination factor Rho motor.. Transcription, Taylor & Francis, 2010, 1 (2), pp.70-75. ⟨10.4161/trns.1.2.12232⟩. ⟨hal-00591655⟩
- Makhlouf Rabhi, Roman Tuma, Marc Boudvillain. RNA remodeling by hexameric RNA helicases. RNA Biology, Taylor & Francis, 2010, 7 (6 - Sp. Iss. SI), pp.655-666. ⟨10.4161/rna.7.6.13570⟩. ⟨hal-00602496⟩
- C. Riedel, R. Arinero, Ph. Tordjeman, M. Ramonda, G. Lévêque, et al.. Determination of the nanoscale dielectric constant by means of a double pass method using electrostatic force microscopy. Journal of Applied Physics, American Institute of Physics, 2009, 106 (2), ⟨10.1063/1.3182726⟩. ⟨hal-01791002⟩
- Annie Schwartz, Makhlouf Rabhi, Frederique Jacquinot, Emmanuel Margeat, A. Rachid Rahmouni, et al.. A stepwise 2 '-hydroxyl activation mechanism for the bacterial transcription termination factor Rho helicase. Nature Structural and Molecular Biology, Nature Publishing Group, 2009, 16 (12), pp.1309-1316. ⟨10.1038/nsmb.1711⟩. ⟨hal-00525325⟩
- Annie Schwartz, Céline Walmacq, A. Rachid Rahmouni, Marc Boudvillain. Noncanonical Interactions in the Management of RNA Structural Blocks by the Transcription Termination Rho Helicase. Biochemistry, American Chemical Society, 2007, 46 (33), pp.9366 - 9379. ⟨10.1021/bi700493m⟩. ⟨hal-00166551⟩
- Annie Schwartz, Emmanuel Margeat, A. Rachid Rahmouni, Marc Boudvillain. Transcription termination factor Rho can displace streptavidin from biotinylated RNA. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2007, 282 (43), pp.31469-31476. ⟨10.1074/jbc.M706935200⟩. ⟨hal-00176284⟩
- C. Walmacq, A.R. Rahmouni, M. Boudvillain. Testing the steric exclusion model for hexameric helicases: substrate features that alter RNA-DNA unwinding by the transcription termination factor Rho. Biochemistry, American Chemical Society, 2006, 45, pp.5885-5895. ⟨10.1021/bi0600648⟩. ⟨hal-00088941⟩
- C. Walmacq, A.R. Rahmouni, M. Boudvillain. Influence of substrate composition on the helicase activity of transcription termination factor Rho: reduced processivity of Rho hexamers during unwinding of RNA-DNA hybrid regions. Journal of Molecular Biology, Elsevier, 2004, 342 (2), pp.403-420. ⟨10.1016/j.jmb.2004.07.026⟩. ⟨hal-00113090⟩
- A. Schwartz, A. Rachid Rahmouni, Marc Boudvillain. The functional anatomy of an intrinsic transcription terminator. EMBO Journal, EMBO Press, 2003, 22 (13), pp.3385 - 3394. ⟨10.1093/emboj/cdg310⟩. ⟨hal-01880611⟩
- Kathrin Schmidt, Marc Boudvillain, Annie Schwartz, Gijs van der Marel, Jacques van Boom, et al.. Monofunctionallytrans-Diammine Platinum(II)-Modified Peptide Nucleic Acid Oligomers: A New Generation of Potential Antisense Drugs. Chemistry - A European Journal, Wiley-VCH Verlag, 2002, 8 (24), pp.5566 - 5570. ⟨10.1002/1521-3765(20021216)8:24<5566::AID-CHEM5566>3.0.CO;2-#⟩. ⟨hal-01880614⟩
- Eva Montero, José Pérez, Annie Schwartz, Miguel Fuertes, Jean-Marc Malinge, et al.. Apoptosis induction and DNA interstrand cross-link formation by cytotoxic trans-[PtCl2(NH(CH3)2)(NHCH(CH3)2) : cross-linking between d(G) and complementary d(C) within oligonucleotide duplexes.. ChemBioChem, Wiley-VCH Verlag, 2002, 3 (1), pp.61-7. ⟨hal-02132063⟩
- Marc Boudvillain, Annie Schwartz, A. Rachid Rahmouni. Limited Topological Alteration of the T7 RNA Polymerase Active Center at Intrinsic Termination Sites †. Biochemistry, American Chemical Society, 2002, 41 (9), pp.3137-3146. ⟨10.1021/bi011358q⟩. ⟨hal-02075291⟩
- Boudvillain Marc, Rozenn Dalbiès-Tran, Marc Leng, Marc Boudvillain. Oligonucleotides Modified With Transplatin Derivatives: Fast and Efficient Metalloribozymes. Metal-Based Drugs, Hindawi Publishing Corporation, 2001, 8 (1), pp.39 - 45. ⟨10.1155/MBD.2001.39⟩. ⟨hal-01880601⟩
- Jens Müller, Markus Drumm, Marc Boudvillain, Marc Leng, Einar Sletten, et al.. Parallel-stranded DNA with Hoogsteen base pairing stabilized by a trans-[Pt(NH3)2]2+ cross-link: characterization and conversion into a homodimer and a triplex. Journal of Biological Inorganic Chemistry, Springer Verlag, 2000, 5 (5), pp.603-611. ⟨10.1007/s007750000143⟩. ⟨hal-02075297⟩
- Françoise Paquet, Marc Boudvillain, Gérard Lancelot, Marc Leng. NMR solution structure of a DNA dodecamer containing a transplatin interstrand GN7-CN3 cross-link. Nucleic Acids Research, Oxford University Press, 1999, 27 (21), pp.4261-4268. ⟨10.1093/nar/27.21.4261⟩. ⟨hal-02075311⟩
- Caroline Colombier, Marc Boudvillain, Marc Leng. Interstrand Crosslinking Reaction in Transplatin-Modified Oligo-2‵-O-Methyl Ribonucleotide-RNA Hybrids. Antisense and Nucleic Acid Drug Development, Mary Ann Liebert, 1997, 7 (4), pp.397-402. ⟨10.1089/oli.1.1997.7.397⟩. ⟨hal-02322135⟩
- Marc Boudvillain, Martine Guerin, Rozenn Dalbiès, Tula Saison-Behmoaras, Marc Leng. Transplatin-Modified Oligo(2‘- O -methyl ribonucleotide)s: A New Tool for Selective Modulation of Gene Expression †. Biochemistry, American Chemical Society, 1997, 36 (10), pp.2925 - 2931. ⟨10.1021/bi962695f⟩. ⟨hal-01880625⟩
- Eloy Bernal-Méndez, Marc Boudvillain, Francisco González-Vílchez, Marc Leng. Chemical Versatility of Transplatin Monofunctional Adducts within Multiple Site-Specifically Platinated DNA †. Biochemistry, American Chemical Society, 1997, 36 (24), pp.7281 - 7287. ⟨10.1021/bi9703148⟩. ⟨hal-01880622⟩
- Marc Boudvillain, C. Prevost, P. Beudaert, M. Leng, R. Lavery, et al.. Distortions of the DNA Double Helix Induced by 1,3-trans-Diamminedichloroplatinum(II)-intrastrand Cross-link: An Internal Coordinate Molecular Modeling Study. Journal of Biomolecular Structure and Dynamics, Taylor & Francis: STM, Behavioural Science and Public Health Titles, 1997, 14 (6), pp.703 - 714. ⟨10.1080/07391102.1997.10508173⟩. ⟨hal-01880624⟩
- Marc Boudvillain, R Dalbiès, M. Leng. Evidences for a catalytic activity of the DNA double helix in the reaction between DNA, platinum(II), and intercalators.. Metal Ions in Biological Systems, Marcel Dekker, 1996, 33, pp.87-104. ⟨hal-02322086⟩
- Rozenn Dalbiès, Marc Boudvillain, Marc Leng. Linkage isomerization reaction of intrastrand cross-links in trans -diamminedichloroplatinum(II)-modified single-stranded oligonucleotides. Nucleic Acids Research, Oxford University Press, 1995, 23 (6), pp.949-953. ⟨10.1093/nar/23.6.949⟩. ⟨hal-02075321⟩
- Marc Boudvillain, Rozenn Dalbiès, Christophe Aussourd, Marc Leng. Intrastrand cross-links are not formed in the reaction between transplatin and native DNA: relation with the clinical inefficency of transplatin. Nucleic Acids Research, Oxford University Press, 1995, 23 (13), pp.2381-2388. ⟨10.1093/nar/23.13.2381⟩. ⟨hal-02075318⟩
- M. Decoville, A. Schwartz, D. Locker, M. Leng. Detection of minor adducts in cisplatin-modified DNA by transcription footprinting.. FEBS Letters, Wiley, 1993, 323 (1-2), pp.55-8. ⟨hal-02119335⟩
- M. Lemaire, A. Rachid Rahmouni, A. Schwartz, M. Leng. Interstrand cross-links are preferentially formed at the d(GC) sites in the reaction between cis-diamminedichloroplatinum (II) and DNA.. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 1991, 88 (5), pp.1982-1985. ⟨10.1073/pnas.88.5.1982⟩. ⟨hal-02127202⟩
- Jean-Marc Malinge, Annie Schwartz, M. Leng. Reaction of nucleic-acids with cis-dichlorodiammineplatinum (II) in the presence of intercalating agents - characterization of new ternary complexes. Bulletin du Cancer, John Libbey Eurotext, 1988, 75 (7), pp.668. ⟨hal-02171328⟩
- Jean-Marc Malinge, Annie Schwartz, Marc Leng. Characterization of the ternary complexes formed in the reaction of cis-diamminedichloro platinum (II), ethidium bromide and nucleic acids. Nucleic Acids Research, Oxford University Press, 1987, 15 (4), pp.1779-1797. ⟨10.1093/nar/15.4.1779⟩. ⟨hal-02134724⟩
- A. Rachid Rahmouni, Jean-Marc Malinge, Annie Schwartz, Marc Leng. Comparison Between poly(dG-dC)·poly(dG-dC) and DNA Modified by cis-diamminedichloroplatinum (II): Immunological and Spectroscopic Studies. Journal of Biomolecular Structure and Dynamics, Taylor & Francis: STM, Behavioural Science and Public Health Titles, 1985, 3 (2), pp.363-375. ⟨10.1080/07391102.1985.10508423⟩. ⟨hal-02127084⟩
Poster9 documents
- E. Eveno, C. Nadiras, N. Figueroa-Bossi, A. Schwartz, M. Boudvillain. Predicting Rho-dependent termination of transcription at genome scale. 24th Annual Meeting of the RNA Society, Jun 2019, Cracovie, Poland. ⟨hal-02902104⟩
- I. Simon, A. Schwartz, M. Delaleau, L. Bossi, N. Figueroa-Bossi, et al.. Exploring Rho helicase activity for the purpose of synthetic regulation. 32e Colloque Biotechnocentre, Oct 2019, Seillac, France. ⟨hal-02902096⟩
- E. Eveno, C. Nadiras, A. Schwartz, N. Figueroa-Bossi, M. Boudvillain. Genome-scale prediction of Rho-dependent termination of transcription. 11e Rencontre SifrARN (SFBBM), Nov 2018, Nancy, France. ⟨hal-02902107⟩
- C. Clerte, O. Manier, J-B. Fiche, M. Boudvillain, L. Bossi, et al.. Relationships between Rho and RNA polymerase in living bacteria. Le Studium conference “Structural biology and biophysics of RNA-protein complexes”, Nov 2017, Orléans, France. ⟨hal-02902111⟩
- C. Nadiras, E. Eveno, M. Boudvillain. Towards a model for predicting Rho-dependent transcription termination sites. Le Studium conference “Structural biology and biophysics of RNA-protein complexes”, Nov 2017, Orléans, France. ⟨hal-02902112⟩
- C. Nadiras, E. Eveno, M. Boudvillain. Identification des sites de terminaison de transcription Rho-dépendantes dans les génomes bactériens. 30e Colloque Biotechnocentre, Oct 2017, Seillac, France. ⟨hal-02902116⟩
- C. Nadiras, E. Eveno, M. Boudvillain. Seeking Rho-dependent transcription termination sites within bacterial genomes. EMBO & Harden conference & Helicases and Nucleic Acid Translocases, Jul 2017, Kloster Banz-Bamberg, Germany. ⟨hal-02902120⟩
- R. Vishwakarma, A. Nord, F. Pedaci, M. Boudvillain, E. Margeat. Single-molecule analysis of E. coli Rho translocase, Helicase, transcription termination activities, and its interactions with RNA polymerase. Le Studium conference “Structural biology and biophysics of RNA-protein complexes”, Nov 2017, Orléans, France. ⟨hal-02902115⟩
- C. Nadiras, Eric Eveno, A. Schwartz, M. Boudvillain. Analyse des terminateurs Rho-dépendants bactériens. 29e colloque Biotechnocentre, Oct 2016, Seillac, France. ⟨hal-02453368⟩
Ouvrage (y compris édition critique et traduction)1 document
- Marc Boudvillain. Methods in Molecular Biology - RNA remodeling proteins. In press, ⟨10.1007/978-1-0716-0935-4⟩. ⟨hal-03036533⟩
Chapitre d'ouvrage10 documents
- Isabelle Simon, Marc Boudvillain. A simple fluorescence microplate assay to monitor RNA-DNA hybrid unwinding by the bacterial transcription termination factor Rho. Marc Boudvillain. RNA remodeling proteins, 2209, Humana press, pp.143-161, In press, Methods in Molecular Biology, ⟨10.1007/978-1-0716-0935-4_10⟩. ⟨hal-03036494⟩
- Cédric Nadiras, Annie Schwartz, Mildred Delaleau, Marc Boudvillain. Evaluating the Effect of Small RNAs and Associated Chaperones on Rho-Dependent Termination of Transcription In Vitro.. Methods in Mecular Biology, 1737, humana press, Totowa, NJ-USA, pp.99-118, 2018, Bacterial regulatory RNA (Arluison & Valverde, eds), ⟨10.1007/978-1-4939-7634-8_7⟩. ⟨hal-01880631⟩
- F. d'Heygère, A. Schwartz, F. Coste, B. Castaing, M. Boudvillain. Monitoring RNA Unwinding by the Transcription Termination Factor Rho from Mycobacterium tuberculosis. Methods in Mecular Biology, 1259, Springer, pp.293-311, 2015, RNA remodelling proteins (Boudvillain, ed), 978-1-4939-2213-0. ⟨10.1007/978-1-4939-2214-7_18⟩. ⟨hal-01174055⟩
- Olga Fedorova, Marc Boudvillain, Christina Waldsich. Nucleotide Analog Interference Mapping and Suppression (NAIM/NAIS): a Combinatorial Approach to Study RNA Structure, Folding, and Interaction with Proteins. Handbook of RNA Biochemistry, 2nd edition (Westhof, Bindereif, Schon & Hartmann, eds), Wiley-VCH Verlag GmbH & Co. KGaA, pp.329-368, 2014, ⟨10.1002/9783527647064.ch17⟩. ⟨hal-02097735⟩
- Makhlouf Rabhi, Rahmouni Rachid A., Marc Boudvillain. Transcription termination factor Rho: a ring-shaped RNA helicase from bacteria. Eckhard Jankowsky. RNA helicases, Royal Society of Chemistry Publishing, pp.243-271, 2010, ⟨10.1039/9781849732215-00243⟩. ⟨hal-00610004⟩
- Marc Boudvillain, Céline Walmacq, Annie Schwartz, Frédérique Jacquinot. Simple enzymatic assays for the in vitro motor activity of transcription termination factor Rho from Escherichia coli. Mohamed M. M. Abdelhaleem. Methods in Mecular Biology, Humana Press, pp.137-154, 2009, Helicases (Abdelhaleem, ed), ⟨10.1007/978-1-60327-355-8_10⟩. ⟨hal-00623571⟩
- O. Fedorova, M. Boudvillain, J. Kawaola, A.M. Pyle. Nucleotide analog interference mapping and suppression (NAIM/NAIS): specific applications in studies of RNA tertiary structure, dynamic helicase mechanism, and RNA-protrein interactions. E. Westhof, A. Bindereif, A. Schon, R.I. Hartmann. Handbook of RNA biochemistry, Wiley-VCH, pp.259-293, 2005. ⟨hal-00088591⟩
- Marc Boudvillain, Dalbiès R., Leng M.. Trans-Diamminedichloroplatinum (II) Is Not an Antitumor Drug: Why?. Platinum and Other Metal Coordination Compounds in Cancer Chemotherapy, 2, Plenum Press, New'Tork, USA, pp.17-25, 1996. ⟨hal-02097755⟩
- Frederic Gaucheron, Marie-Agnes Lemaire, Jean-Marc Malinge, Annie Schwartz, Marc Leng. Reactivity of DNA and cis-Diamminedichloroplatinumul(II) in the Presence of Intercalating Agents. Platinum and Other Metal Coordination Compounds in Cancer Chemotherapy, Springer US, pp.73-79, 1991, 978-1-4899-0740-0. ⟨hal-02134699⟩
- A. Rachid Rahmouni, A. Schwartz, M. Leng. Importance of DNA Sequence in the Reaction of d(ApG) and d(GpA) with Cis-Diamminedichloroplatinum (II). Platinum and Other Metal Coordination Compounds in Cancer Chemotherapy: Proceedings of the Fifth International Symposium on Platinum and Other Metal Coordination Compounds in Cancer Chemotherapy Abano, Padua, ITALY - June 29–July 2, 1987, Springer US, pp.127-131, 1988. ⟨hal-02127164⟩
Brevet1 document
- Marc Leng, Rozenn Dalbiès, Marc Boudvillain. Procédé de pontage sélectif et irréversible d'oligonucléotides platinés sur de l'ARN et ses applications. France, Patent n° : WO1998018915A2. 1996. ⟨hal-02097839⟩